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Clinical and Diagnostic Laboratory Immunology, May 2003, p. 383-387, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.383-387.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Humoral Immune Responses to S-Layer-Like Proteins of Bacteroides forsythus

Masahiro Yoneda,1* Takao Hirofuji,2 Noriko Motooka,1 Koji Nozoe,1 Kayoko Shigenaga,3 Hisashi Anan,1 Mayumi Miura,1 Hiroaki Kabashima,1 Akiko Matsumoto,1 and Katsumasa Maeda1

Section of Periodontology, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University,1 Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College,2 Department of Medicine, Fukuoka Prison, Fukuoka, Japan3

Received 7 October 2002/ Returned for modification 8 February 2003/ Accepted 22 February 2003

Bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an S-layer outside the outer membrane. The S-layer-like antigens were recently isolated from B. forsythus, and they were found to be 270- and 230-kDa proteins in the envelope fraction. In this study, these proteins were confirmed to be specific to B. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adult and early-onset periodontitis in Western immmunoblot analysis. We compared the immunoglobulin G (IgG) responses against the purified S-layer-like antigen by enzyme-linked immunosorbent assay. IgG responses against this antigen were low in healthy control subjects, but they were significantly higher in subjects with adult and early-onset periodontitis. Together with the fact that the IgG responses against the crude extract of B. forsythus did not rise significantly in patients with periodontitis, S-layer-like proteins are considered to be specific antigens of B. forsythus and may play an important role in the progression of periodontitis.

* Corresponding author. Mailing address: Section of Periodontology, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-6358. Fax: 81-92-642-6360. E-mail: yoneda{at}dent.kyushu-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 383-387, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.383-387.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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