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Clinical and Diagnostic Laboratory Immunology, May 2003, p. 476-478, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.476-478.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples

F. Nato,1* A. Boutonnier,1 M. Rajerison,2 P. Grosjean,2 S. Dartevelle,1 A. Guénolé,1 N. A. Bhuiyan,3 D. A. Sack,3 G. B. Nair,3 J. M. Fournier,1 and S. Chanteau2,{dagger}

Institut Pasteur, Paris, France,1 Institut Pasteur, Antananarivo, Madagascar,2 Laboratory Sciences Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh3

Received 24 October 2002/ Returned for modification 10 December 2002/ Accepted 28 January 2003

We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.

* Corresponding author. Mailing address: Institut Pasteur, PT5, Département de Biologie Structurale et Chimie, 25-28, Rue du Dr. Roux, 75015 Paris, France. Phone: 33 1 45 68 87 98. Fax: 33 1 40 61 39 70. E-mail: fnato{at}pasteur.fr.

{dagger} Present address: CERMES, Niamey, Niger.

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 476-478, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.476-478.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Bhuiyan, N. A., Qadri, F., Faruque, A. S. G., Malek, M. A, Salam, M. A., Nato, F., Fournier, J. M., Chanteau, S., Sack, D. A., Balakrish Nair, G. (2003). Use of Dipsticks for Rapid Diagnosis of Cholera Caused by Vibrio cholerae O1 and O139 from Rectal Swabs. J. Clin. Microbiol. 41: 3939-3941 [Abstract] [Full Text]  


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