This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prince, H. E.
Right arrow Articles by Leber, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prince, H. E.
Right arrow Articles by Leber, A. L.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 481-482, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.481-482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Complement Fixation and Hemagglutination Inhibition Assays for Detecting Antibody Responses following Influenza Virus Vaccination

Harry E. Prince1* and Amy L. Leber2

Focus Technologies, Cypress, California,1 Quest Diagnostics, San Juan Capistrano, California2

Received 23 December 2002/ Returned for modification 13 February 2003/ Accepted 4 March 2003

Complement fixation (CF) was compared to hemagglutination inhibition (HI) as a method for identifying antibody responses to influenza virus vaccination. CF assays were performed at two different laboratories using paired (pre- and postvaccination) sera from 38 vaccinated laboratory employees; HI assays were performed at a third laboratory. As expected, most vaccinees (31/38 = 82%) responded to at least one of three influenza virus antigens as measured by HI. In contrast, only 21% (8/38) of vaccinees showed a response by CF at laboratory 1, and only 29% (11/38) showed a response by CF at laboratory 2. These findings indicate that due to low sensitivity, CF assays should not be used to assess the antibody response to influenza virus vaccination.

* Corresponding author. Mailing address: Focus Technologies, 5785 Corporate Ave., Cypress, CA 90630. Phone: (714) 220-1900. Fax: (714) 484-1296. E-mail: hprince{at}focusanswers.com.

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 481-482, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.481-482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»