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Clinical and Diagnostic Laboratory Immunology, July 2003, p. 552-557, Vol. 10, No. 4
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.4.552-557.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein

Tetsuro Ikegami,^1,2 Masahiro Niikura,^1, Masayuki Saijo,¹ Mary E. Miranda,³ Alan B. Calaor,³ Marvin Hernandez,³ Luz P. Acosta,³ Daria L. Manalo,³ Ichiro Kurane,¹ Yasuhiro Yoshikawa,² and Shigeru Morikawa^1*

Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011,¹ Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan,² Veterinary Research Department, Research Institute for Tropical Medicine, Department of Health, Muntinlupa City 1770, Philippines³

Received 21 February 2003/ Returned for modification 3 April 2003/ Accepted 5 May 2003

Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

Present address: USDA-ARS Avian Disease and Oncology Laboratory, East Lansing, MI 48823.