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Clinical and Diagnostic Laboratory Immunology, July 2003, p. 622-630, Vol. 10, No. 4
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.4.622-630.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Capture Immunoglobulin M (IgM) and IgG Enzyme-Linked Immunosorbent Assay (ELISA) and Nonstructural Protein NS1 Serotype-Specific IgG ELISA for Differentiation of Primary and Secondary Dengue Virus Infections

Pei-Yun Shu,1 Li-Kuang Chen,2 Shu-Fen Chang,1 Yi-Yun Yueh,1 Ling Chow,1 Li-Jung Chien,1 Chuan Chin,3 Ting-Hsiang Lin,1 and Jyh-Hsiung Huang1*

Division of Research Development and Laboratory Diagnosis, Center for Disease Control, Department of Health,1 Co-wealth Medical Science and Biotechnology, Taipei,3 Department of Emergency Medicine, Medical Center, Tzu Chi College of Medicine, Hualien, Taiwan, Republic of China2

Received 13 January 2003/ Returned for modification 17 March 2003/ Accepted 1 April 2003

We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due to the fact that the NS1-specific IgG antibody cannot be detected before day 9 of illness for primary infection, so the NS1-specific IgG antibodies measured in acute-phase sera must come from previous infection. Comparison of NS1 serotype-specific IgG ELISA with envelope- and membrane-specific capture IgM and IgG ELISA in the differentiation of primary and secondary dengue virus infections showed good correlation (95.90% agreement). Most important, we have found that the serotype of the dengue virus from the majority of patients with primary infection could be correctly identified when convalescent-phase or postinfection sera were analyzed by NS1 serotype-specific IgG ELISA. These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection.

* Corresponding author. Mailing address: Division of Research Development and Laboratory Diagnosis, Center for Disease Control, Department of Health, 161, Kun-Yang St., Taipei, Taiwan, Republic of China. Phone: 886-2-26531374. Fax: 886-2-27883992. E-mail: jhhuang{at}cdc.gov.tw.

Clinical and Diagnostic Laboratory Immunology, July 2003, p. 622-630, Vol. 10, No. 4
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.4.622-630.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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