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Clinical and Diagnostic Laboratory Immunology, September 2003, p. 882-885, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.882-885.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Serum Glucan Levels Are Not Specific for Presence of Fungal Infections in Intensive Care Unit Patients

Justin Digby, John Kalbfleisch, Andy Glenn, Angie Larsen, William Browder, and David Williams*

Departments of Surgery and Medical Education, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 and,1 James H. Quillen Veterans Affairs Medical Center, Mountain Home, Tennessee 376842

Received 24 February 2003/ Returned for modification 31 March 2003/ Accepted 7 July 2003

Fungal infections in the critically ill patient are difficult to diagnose and are associated with a high mortality rate. A major obstacle to managing fungal infection is the lack of a reliable clinical assay that will rapidly identify patients with fungal sepsis. Glucans are polymers of glucose that are found in the cell wall of fungi and certain bacteria. Glucans are also released from the fungal cell wall into the extracellular milieu. Several studies have reported that detection of fungal glucan in serum or plasma is useful in the diagnosis of mycoses. However, recent studies have questioned the clinical utility of this assay. In this study, we examined serum glucan levels in intensive care unit (ICU) patients and attempt to correlate serum glucan levels with the presence of fungal infection. Following attainment of informed consent, serum was harvested from 46 ICU patients with confirmed fungal infections, confirmed bacterial infections, or no evidence of infection. Sera from eight healthy volunteers served as control. Serum glucan was assayed with a glucan-specific Limulus assay. Serum glucan levels were increased (69.6 ± 17 pg/ml; P < 0.001) in ICU patients versus the normal (11.5 ± 1.3 pg/ml) and noninfected ICU (27.4 ± 17 pg/ml) controls. However, serum glucan levels were not different in patients with confirmed fungal infections versus those with confirmed bacterial infections. Thus, serum glucan levels did not show a correlation with the presence of fungal infections and do not appear to be specific for fungal infections. However, the assay may be useful as a negative predictor of infection.

* Corresponding author. Mailing address: Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, P.O. Box 70575, Johnson City, TN 37614-0575. Phone: (423) 439-6363. Fax: (423) 439-6259. E-mail: williamd{at}etsu.edu.

Clinical and Diagnostic Laboratory Immunology, September 2003, p. 882-885, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.882-885.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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