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Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1037-1042, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1037-1042.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Variation at the Mitochondrial Large-Subunit rRNA Locus of Pneumocystis Isolates from Simian Immunodeficiency Virus-Infected Rhesus Macaques

Karen A. Norris,* Hans Wildschutte, Jennifer Franko, and Kathryn F. Board

Department of Immunology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

Received 23 January 2003/ Returned for modification 24 April 2003/ Accepted 25 July 2003

The nucleotide sequences of a segment of the Pneumocystis mitochondrial large-subunit (mt LSU) rRNA gene from rhesus macaques coinfected with simian immunodeficiency virus (SIV) and Pneumocystis carinii were examined. Of 12 isolates examined, 3 were found to be identical and the others showed substantial sequence variation, with up to 13% divergence among variants. We identified two general sequence types that differed at several sites, including a conserved 26-nucleotide insertion. Four monkeys had evidence of two Pneumocystis variants present simultaneously, indicative of a mixed infection. There was a high degree of variance between the rhesus macaque-derived Pneumocystis mt LSU rRNA gene sequence and the cognate sequences in Pneumocystis organisms derived from other hosts. Analysis of the mt LSU rRNA genes of Pneumocystis organisms derived from rhesus macaques and several other mammalian hosts supports the observation that rhesus macaque-derived Pneumocystis is most closely related to human-derived Pneumocystis. In addition, the data identify the mt LSU rRNA gene as an informative locus for transmission and epidemiological studies of the SIV-rhesus macaque model of Pneumocystis infection.

* Corresponding author. Mailing address: Department of Immunology, University of Pittsburgh School of Medicine, E 1038 Biomedical Science Tower, Pittsburgh, PA 15261. Phone: (412) 648-8848. Fax: (412) 383-8096. E-mail: kan1{at}pitt.edu.

Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1037-1042, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1037-1042.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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