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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 42-49, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.42-49.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Performance and Use of a Ribonucleotide Reductase Herpes Simplex Virus Type-Specific Serological Assay

S. Q. Wales,1,2 C. C. Smith,2 M. Wachsman,1 G. Calton,1 and L. Aurelian2*

AuRx, Inc., Glen Burnie, Maryland 21061,1 Virology/Immunology Laboratories, Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 212012

Received 9 May 2003/ Returned for modification 31 July 2003/ Accepted 25 September 2003

In response to the increasingly evident need for herpes simplex virus (HSV) serotype-specific serologic assays that rely on proteins other than glycoprotein-G (gG), we developed a rapid serologic assay that is based on type-specific epitopes within the large subunit of HSV ribonucleotide reductase (R1). The assay (Au-2 enzyme-linked immunosorbent assay [ELISA]) uses an HSV type 2 (HSV-2) R1 peptide antigen. It provides a reliable method for detecting serotype-specific antibody to a protein other than gG-2. The Au-2 ELISA has high sensitivity and specificity as determined by direct comparison to Western blotting, a widely accepted "gold standard," and to ELISA with an HSV-1 R1 peptide (Au-1). The use of the Au-2 ELISA in conjunction with the gG-2-based assays will improve the sensitivity and specificity of serologic diagnosis and patient management.

* Corresponding author. Mailing address: Virology/Immunology Laboratories, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-3895. Fax: (410) 706-2513. E-mail: laurelia{at}umaryland.edu.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 42-49, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.42-49.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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