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Clinical and Diagnostic Laboratory Immunology, Jul 1995, 499-502, Vol 2, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Bioactive tumor necrosis factor in the sputa of cystic fibrosis patients

N Kelly, S Clee and B Nakielna
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.

Eighty sputum samples from 37 cystic fibrosis patients were assayed for tumor necrosis factor alpha (TNF-alpha) in the L929 bioactivity assay. In addition, the presence of TNF-alpha-specific mRNA in the sputum was determined by using TNF-alpha-specific DNA probes in a PCR amplification step after reverse transcription of the mRNA to cDNA. Bioactive TNF-alpha was detected in 69 (86%) of the 80 sputum samples tested and, on at least one occasion, in 35 (95%) of the 37 cystic fibrosis patients sampled. The highest levels of TNF-alpha were observed in sputa obtained from patients presenting with an exacerbation of their disease.




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