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Clinical and Diagnostic Laboratory Immunology, 01 1997, 11-13, Vol 4, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule

DS Blunt, NV Khramtsov, SJ Upton and BA Montelone
Division of Biology, Kansas State University, Manhattan 66506-4901, USA.

We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low- molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa.


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