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Clinical and Diagnostic Laboratory Immunology, 09 1997, 615-619, Vol 4, No. 5
FR Rurangirwa, PS Shompole, AN Wambugu, SM Kihara and TC McGuire
Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma
capricolum subsp. capripneumoniae in Western blots identifying an epitope
on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates
belonging to four other Mycoplasma species or subspecies closely related to
M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of
intact organisms and detergent- phase partitioning revealed p24 to be an
integral M. capricolum subsp. capripneumoniae surface membrane protein.
Copyright © 1997 by the American Society for Microbiology. All rights reserved.
Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
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