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 Previous Article

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 121-124, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Monoclonal Antibody Specific to Surface Antigen on Candida krusei

Raymond Robert,1,* Odile Faure,2 Arnaud Carloti,3 Bernadette Lebeau,2 Christian Bernard,4 Agnès Marot-Leblond,1 Renée Grillot,2 and Jean-Marcel Senet1

Département de Mycologie-Immunologie-Parasitologie, Faculté de Pharmacie 49100 Angers,1 Département de Parasitologie-Mycologie Médicale et Moléculaire, Faculté de Médecine de Grenoble, Université Joseph Fournier, 38706 La Tronche Cedex,2 Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Faculté de Pharmacie, Université Claude Bernard-Lyon 1, 69373 Lyon Cedex 08,3 and SR2B, PAT Carrière Beurrière, 49240 Avrillé,4 France

Received 9 June 1997/Returned for modification 29 August 1997/Accepted 27 October 1997

A monoclonal antibody (MAb; MAb 6B3) which reacts specifically with a cell wall antigen found in all strains or isolates of Candida krusei was developed. MAb 6B3 was extensively tested by immunofluorescence assay for cross-reaction with many Candida, Cryptococcus, Saccharomyces, Trichosporon, and Rhodotorula species and was found to react only with the species C. krusei. The specific epitope is expressed on the surface of fungal cells and appears to reside on a protein moiety. Taking into account the increasing importance of fluconazole-resistant strains in nosocomial fungal infections, the very high degree of specificity of this MAb for C. krusei could be useful for the routine detection of C. krusei in culture or in tissue samples.

* Corresponding author. Mailing address: Laboratoire de Mycologie, Faculté de Pharmacie, 16 boulevard Daviers 49100, Angers, France. Phone: 332-41-22-66-60. Fax: 332-41-48-67-33. E-mail: raymond.robert{at}univ-angers.fr.

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 121-124, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Marot-Leblond, A., Grimaud, L., David, S., Sullivan, D. J., Coleman, D. C., Ponton, J., Robert, R. (2004). Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis. J. Clin. Microbiol. 42: 4956-4960 [Abstract] [Full Text]  
  • Marot-Leblond, A., Grimaud, L., Nail, S., Bouterige, S., Apaire-Marchais, V., Sullivan, D. J., Robert, R. (2000). New Monoclonal Antibody Specific for Candida albicans Germ Tube. J. Clin. Microbiol. 38: 61-67 [Abstract] [Full Text]  


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