This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barber, M. R.
Right arrow Articles by Yang, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barber, M. R.
Right arrow Articles by Yang, T. J.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 82-86, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Chemotactic Activities in Nonmastitic and Mastitic Mammary Secretions: Presence of Interleukin-8 in Mastitic but Not Nonmastitic Secretions

Michele R. Barber and T. J. Yang*

Department of Pathobiology, University of Connecticut, Storrs, Connecticut

Received 8 August 1997/Returned for modification 22 September 1997/Accepted 23 October 1997

Due to its association with low-quality milk and a decrease in milk production in bovines, mastitis is a major cause of economic loss. Additionally, mastitis can be harmful to suckling newborns and can cause damage to the mammary gland. In mastitic mammary secretions there is a substantial increase in somatic cells, specifically neutrophils. In this study we examined the ability of mastitic and nonmastitic mammary secretions to cause in vitro neutrophil chemotaxis using a microchemotaxis assay. Also, the role of the inflammatory chemokine interleukin-8 (IL-8) in neutrophil recruitment during mastitis was addressed in these in vitro experiments. We found that both nonmastitic and mastitic mammary secretions were chemotactic, not chemokinetic, for neutrophils. The neutrophil chemotactic activity in mastitic, but not nonmastitic, mammary secretions was blocked by anti-IL-8 antibodies. Molecular mass separation of the active components showed that the chemotactic activity of the mastitic secretions was present in the 10-kDa-or-less fraction and was blocked by anti-IL-8 antibodies. These results indicate that IL-8 plays a major role in neutrophil recruitment during mastitis. An understanding of its role will be of help in designing strategies for immunomodulatory therapies for mastitis.

* Corresponding author. Mailing address: The University of Connecticut, Department of Pathobiology, U-89, Storrs, CT 06269-3089. Phone: (860) 486-3739. Fax: (860) 486-2794. E-mail: TYang{at}UConnVM.UConn.Edu.

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 82-86, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vangroenweghe, F., Duchateau, L., Boutet, P., Lekeux, P., Rainard, P., Paape, M. J., Burvenich, C. (2005). Effect of Carprofen Treatment Following Experimentally Induced Escherichia coli Mastitis in Primiparous Cows. J DAIRY SCI 88: 2361-2376 [Abstract] [Full Text]  
  • Vangroenweghe, F., Rainard, P., Paape, M., Duchateau, L., Burvenich, C. (2004). Increase of Escherichia coli Inoculum Doses Induces Faster Innate Immune Response in Primiparous Cows. J DAIRY SCI 87: 4132-4144 [Abstract] [Full Text]  
  • Kuroishi, T., Komine, K.-i., Asai, K.-i., Kobayashi, J., Watanabe, K., Yamaguchi, T., Kamata, S.-i., Kumagai, K. (2003). Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells. CVI 10: 1011-1018 [Abstract] [Full Text]  
  • Diez-Fraile, A., Meyer, E., Duchateau, L., Burvenich, C. (2003). L-Selectin and {beta}2-Integrin Expression on Circulating Bovine Polymorphonuclear Leukocytes During Endotoxin Mastitis. J DAIRY SCI 86: 2334-2342 [Abstract] [Full Text]  
  • Boulanger, D., Bureau, F., Melotte, D., Mainil, J., Lekeux, P. (2003). Increased Nuclear Factor {kappa}B Activity in Milk Cells of Mastitis-Affected Cows. J DAIRY SCI 86: 1259-1267 [Abstract] [Full Text]  
  • Prin-Mathieu, C., Le Roux, Y., Faure, G. C., Laurent, F., Bene, M. C., Moussaoui, F. (2002). Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide. CVI 9: 812-817 [Abstract] [Full Text]  
  • Riollet, C., Rainard, P., Poutrel, B. (2000). Differential Induction of Complement Fragment C5a and Inflammatory Cytokines during Intramammary Infections with Escherichia coli and Staphylococcus aureus. CVI 7: 161-167 [Abstract] [Full Text]  
  • Barber, M. R., Pantschenko, A. G., Hinckley, L. S., Yang, T. J. (1999). Inducible and Constitutive In Vitro Neutrophil Chemokine Expression by Mammary Epithelial and Myoepithelial Cells. CVI 6: 791-798 [Abstract] [Full Text]  
  • Semba, R. D., Kumwenda, N., Taha, T. E., Hoover, D. R., Lan, Y., Eisinger, W., Mtimavalye, L., Broadhead, R., Miotti, P. G., Van Der Hoeven, L., Chiphangwi, J. D. (1999). Mastitis and Immunological Factors in Breast Milk of Lactating Women in Malawi. CVI 6: 671-674 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»