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Clinical and Diagnostic Laboratory Immunology, March 1998, p. 225-229, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Lipopolysaccharide from Nonvirulent Ara+ Burkholderia pseudomallei Isolates Is Immunologically Indistinguishable from Lipopolysaccharide from Virulent Araminus Clinical Isolates

Narisara Anuntagool,1 Pakamas Intachote,1 Vanaporn Wuthiekanun,2 Nicholas J. White,2,3 and Stitaya Sirisinha1,4,*

Laboratory of Immunology, Chulabhorn Research Institute,1 Department of Microbiology, Faculty of Science,4 and Faculty of Tropical Medicine,2 Mahidol University, Bangkok, Thailand, and Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom3

Received 14 July 1997/Returned for modification 21 October 1997/Accepted 15 December 1997

Different lines of evidence suggest that a discrepancy between the distribution of Burkholderia (Pseudomonas) pseudomallei in the environment and the distribution of the disease melioidosis is attributable, at least in part, to phenotypic differences between clinical and some environmental isolates. Two antigenically and biochemically distinct biotypes have been described, only one of which is virulent. In this study, lipopolysaccharides (LPSs) were extracted by the proteinase K digestion method from a total of 214 B. pseudomallei isolates, and their immunoreactivities with sera from patients with different clinical spectra and with other infections were evaluated. With the exception of 4 isolates from a total of 214 tested, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis silver-staining profiles of the LPSs from the two biotypes showed identical ladder patterns that were typical for smooth LPSs from other gram-negative bacteria. The 210 isolates with typical LPS patterns (119 Ara- clinical, 13 Ara- soil, 70 Ara+ soil, and 8 reference National Type Culture Collection strains) also exhibited similar immunoblot profiles against pooled sera from patients with melioidosis and hyperimmune mouse sera. Concordant findings were noted in the indirect enzyme-linked immunosorbent assay with Ara- and Ara+ LPSs to coat the microtiter plates. The LPSs of the different B. pseudomallei biotypes appear antigenically indistinguishable. It is, therefore, unlikely that this component is related to the virulence and pathogenicity of B. pseudomallei.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand. Phone: 662 246 2358, ext. 6606. Fax: 662 644 5411. E-mail: scssr{at}mahidol.ac.th.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 225-229, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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