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Clinical and Diagnostic Laboratory Immunology, March 1998, p. 256-258, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR Detection of Human Cytomegalovirus DNA in Clinical Specimens Using Novel UL37 Exon 3 and US3 Primers

Marci M. Lesperance,1,2,dagger Despina G. Contopoulos-Ioannidis,1,3 María del Pilar Gutiérrez,1,3 and Anamaris M. Colberg-Poley1,*

Center for Virology, Immunology, and Infectious Disease Research,1 Department of Otolaryngology,2 and Department of Infectious Diseases,3 Children's National Medical Center, Washington, D.C. 20010

Received 15 August 1997/Returned for modification 20 October 1997/Accepted 18 November 1997

The sensitivity and specificity of novel UL37 exon 3 (UL37x3) and US3 immediate-early (IE) gene PCR primers to detect human cytomegalovirus (HCMV) DNA in clinical specimens are comparable to those of HCMV DNA polymerase (UL54) primers. The use of these IE primers increases the diagnostic performance of HCMV PCR.

* Corresponding author. Mailing address: Center I Research, Room 5720, Children's National Medical Center, 111 Michigan Ave., NW, Washington, D.C. 20010. Phone: (202) 884-3984. Fax: (202) 884-3985. E-mail:colberam{at}gwis2.circ.gwu.edu.

dagger Present address: Department of Otolaryngology---Head and Neck Surgery, The University of Michigan Medical Center, C. S. Mott Hospital, Ann Arbor, MI 48109-0241.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 256-258, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 1998 by the American Society for Microbiology. All rights reserved.