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Clinical and Diagnostic Laboratory Immunology, July 1998, p. 561-566, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Assignment of Immunoglobulin G1 and G2 Concentrations to Pneumococcal Capsular Polysaccharides 3, 6B, 14, 19F, and 23F in Pneumococcal Reference Serum 89-SF

Anu Soininen,^1,* Ilkka Seppälä,² Tomi Wuorimaa,¹ and Helena Käyhty¹

Department of Vaccines, National Public Health Institute (KTL),¹ and Infection Serology Unit, Division of Bacteriology and Immunology, HUCH Diagnostics, Helsinki University Central Hospital,² Helsinki, Finland

Received 23 December 1997/Returned for modification 26 February 1998/Accepted 22 April 1998

We describe standardization of an enzyme-linked immunosorbent assay (ELISA) for measuring immunoglobulin G1 (IgG1) and IgG2 concentrations of antibodies to pneumococcal capsular polysaccharide (Pnc PS). The ELISA uses a human pneumococcal reference serum pool, lot 89-SF, as a reference. IgG1 and IgG2 concentrations were assigned to antibodies to Pnc PS serotypes 3, 6B, 14, 19F, and 23F in 89-SF by ELISA using affinity-purified monoisotypic IgG1 and IgG2 preparations. The sum of IgG1 and IgG2 concentrations in 89-SF agrees well with the previously assigned IgG concentrations. The IgG1 and IgG2 values in 89-SF were used to measure antibodies to Pnc PS 6B, 14, and 23F in adult pre- and postimmunization sera and the sum of IgG1 and IgG2 concentrations correlated well with the IgG values. Furthermore, the IgG2/IgG1 ratio did not affect the detection of IgG1, the isotype usually represented by a lower concentration.

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^* Corresponding author. Mailing address: National Public Health Institute, Department of Vaccines, Laboratory of Vaccine Immunology, Mannerheimintie 166, 00300 Helsinki, Finland. Phone: 358-9-4744 8587. Fax: 358-9-4744 8599. E-mail: anu.soininen{at}ktl.fi.

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Clinical and Diagnostic Laboratory Immunology, July 1998, p. 561-566, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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