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Clinical and Diagnostic Laboratory Immunology, July 1998, p. 588-591, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Inhibition of Mycobacterium bovis BCG-Induced Tumor Necrosis Factor Alpha Secretion in Human Cells by Transforming Growth Factor beta

Patricia Méndez-Samperio,* Marisol Hernandez-Garay, and Angela Nuñez Vazquez

Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, México, D.F. 11340 México

Received 20 October 1997/Returned for modification 21 January 1998/Accepted 20 March 1998

The effect of exogenous transforming growth factor beta  (TGF-beta ) on Mycobacterium bovis BCG-induced tumor necrosis factor alpha (TNF-alpha ) production by human mononuclear cells was studied. It was found that TNF-alpha production by human cells stimulated with BCG was significantly inhibited by TGF-beta . The specificity of the observed inhibition was demonstrated, since the addition of an anti-TGF-beta neutralizing monoclonal antibody completely reversed the inhibitory effect. Furthermore, the suppressive effect of TGF-beta on TNF-alpha secretion in this system was not due to a direct cytotoxic effect, since cell viability was comparable in the presence or absence of TGF-beta . Interestingly, our results demonstrated comparative suppressive effects of TGF-beta and interleukin-10 on BCG-induced TNF-alpha secretion. Together, the data demonstrate, for the first time, that TGF-beta inhibits BCG-induced TNF-alpha secretion by human cells.

* Corresponding author. Mailing address: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, México, D.F. 11340, México. Phone: 729-6000, ext. 62364. Fax: (5) 396 35 03.

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 588-591, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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