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Clinical and Diagnostic Laboratory Immunology, September 1998, p. 627-631, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Human Toxoplasma-Specific Immunoglobulins A, M, and G with a Recombinant Toxoplasma gondii Rop2 Protein

Valentina Martin,1 Miriam Arcavi,2 Graciela Santillan,1 Maria Regina R. Amendoeira,3 Elizabeth De Souza Neves,4 Gloria Griemberg,2 Eduardo Guarnera,1 Juan C. Garberi,1 and Sergio O. Angel1,*

Departamento de Parasitología, ANLIS Dr. Carlos G. Malbran,1 and Immunología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas José de San Martin-UBA,2 Buenos Aires, Argentina, and Instituto Oswaldo Cruz-Fundaçao Oswaldo Cruz,3 and Hospital Evandro Chagas-FIOCRUZ,4 Rio de Janeiro, Brazil

Received 14 January 1998/Returned for modification 25 February 1998/Accepted 20 May 1998

The Toxoplasma gondii rhoptry protein Rop2 was expressed in Escherichia coli as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2196-561). Humoral response during Toxoplasma infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2196-561 as the antigen substrate. The analyzed sera were divided according to T. gondii-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG+ IgA- IgM-; n = 35), group B (IgG+ IgA+ IgM+; n = 21), group C (IgG+ IgA+ IgM-; n = 5), and group D (IgG+ IgA- IgM+; n = 16). Twenty-six T. gondii-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2196-561 was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since T. gondii Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2196-561 would be suitable for use in diagnostic systems, in combination with other T. gondii antigens, to detect specific IgG, IgA, and IgM antibodies.

* Corresponding author. Mailing address: Departamento de Parasitología, ANLIS Dr. Carlos G. Malbran, Av. Velez Sarsfield 563, 1281 Buenos Aires, Argentina. Phone: 541 301 7437. Fax: 541 303 2382. E-mail: guarnera{at}mail.retina.ar.

Clinical and Diagnostic Laboratory Immunology, September 1998, p. 627-631, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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