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Clinical and Diagnostic Laboratory Immunology, March 1999, p. 269-272, Vol. 6, No. 2
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis

C. M. Marín,1 E. Moreno,2 I. Moriyón,3,* R. Díaz, and J. M. Blasco1

Unidad de Sanidad Animal, Servicio de Investigación Agraria, Diputación General de Aragón, Zaragoza,1 and Departamento de Microbiología, Universidad de Navarra, Pamplona,3 Spain, and Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica2

Received 6 July 1998/Returned for modification 9 October 1998/Accepted 23 November 1998

Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis.

* Corresponding author. Mailing address: Departamento de Microbiología, Universidad de Navarra, Aptdo. 177, 31080 Pamplona, Spain. Phone: 34-948-425600. Fax: 34-948-425649. E-mail: imoriyon{at}unav.es.

Clinical and Diagnostic Laboratory Immunology, March 1999, p. 269-272, Vol. 6, No. 2
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Munoz, P. M., Marin, C. M., Monreal, D., Gonzalez, D., Garin-Bastuji, B., Diaz, R., Mainar-Jaime, R. C., Moriyon, I., Blasco, J. M. (2005). Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9. CVI 12: 141-151 [Abstract] [Full Text]  


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