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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 630-632, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Novel Enzyme-Linked Immunosorbent Assay Using the Recombinant Actinobacillus pleuropneumoniae ApxII Antigen for Diagnosis of Pleuropneumonia in Pig Herds

Gero Leiner,1 Burkart Franz,2,* Katrin Strutzberg,3 and Gerald-F. Gerlach1

Institut für Mikrobiologie und Tierseuchen1 and Institut für Biometrie, Epidemiologie und Informationsverarbeitung,2 Tierärztliche Hochschule Hannover, and IVD GmbH,3 30173 Hannover, Germany

Received 20 November 1998/Returned for modification 10 February 1999/Accepted 26 April 1999

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.


* Corresponding author. Mailing address: Institut für Biometrie, Epidemiologie und Informationsverarbeitung, Tierärztliche Hochschule Hannover, 30173 Hannover, Germany. Phone: 49-511-953 7973. Fax: 49-511-953 7974. E-mail: bfranz{at}micro.tiho-hannover.de.


Clinical and Diagnostic Laboratory Immunology, July 1999, p. 630-632, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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