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Clinical and Diagnostic Laboratory Immunology, September 1999, p. 725-728, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A New Method with General Diagnostic Utility for the Calculation of Immunoglobulin G Avidity

Maria H. Korhonen,1 John Brunstein,1 Heikki Haario,2,3 Alexei Katnikov,3 Roberto Rescaldani,4 and Klaus Hedman1,*

Haartman Institute, Department of Virology,1 and Department of Mathematics,2 University of Helsinki, and ProfMath Ltd.,3 Helsinki, Finland, and Ospedale S. Gerardo Di Monza, Monza, Italy4

Received 22 February 1999/Returned for modification 13 April 1999/Accepted 16 June 1999

The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and Toxoplasma gondii. This approach for IgG avidity calculation is generally applicable and attains the diagnostic performance of the reference method while being less laborious and twice as cost-effective.

* Corresponding author. Mailing address: Haartman Institute and HUCH Diagnostic, Department of Virology, P.O. Box 21 (Haartmaninkatu 3), FIN-00014 University of Helsinki, Helsinki, Finland. Phone: 358-9-1912 6473. Fax: 358-9-1912 6491. E-mail: klaus.hedman{at}helsinki.fi.

Clinical and Diagnostic Laboratory Immunology, September 1999, p. 725-728, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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