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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 106-110, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Two Rapid Diagnostic Assays for Detection of Immunoglobulin M Antibodies to Dengue Virus

Shuenn-Jue L. Wu,1,* Helene Paxton,2 Barbara Hanson,2 Cheryl G. Kung,1 Timothy B. Chen,1 Cindy Rossi,3 David W. Vaughn,4 Gerald S. Murphy,1 and Curtis G. Hayes1

Viral and Rickettsial Diseases Department, Naval Medical Research Center, Bethesda, Maryland 20889-56071; Integrated Diagnostics Inc., Baltimore, Maryland 212272; Diagnostic Systems Division, U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-50113; and Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20307-51004

Received 25 June 1999/Returned for modification 4 August 1999/Accepted 18 October 1999

Two easy-to-use commercial diagnostic assays, a dipstick enzyme-linked immunosorbent assay (ELISA) (Integrated Diagnostics, Baltimore, Md.) and an immunochromatographic card assay (PanBio, Brisbane, Australia) were evaluated for detection of immunoglobulin M (IgM) antibody to dengue virus with an in-house IgM antibody capture microplate ELISA as a reference assay. The dipstick ELISA was based on the indirect-ELISA format using dengue 2 virus as the only antigen and enzyme-labeled goat anti-human IgM antibody as the detector. The total assay time was 75 min. The immunochromatographic card assay was based on the antibody capture format and separately measured both anti-dengue virus IgM and IgG in the same test. Colloidal-gold-labeled anti-dengue virus monoclonal antibody bound with dengue virus 1 to 4 antigen cocktail was the detector, and anti-human IgM and IgG were the capture antibodies. The total assay time was <10 min. Sera from 164 individuals classified as either anti-dengue virus IgM positive (94) or anti-dengue virus IgM negative (70) in the reference microplate ELISA with a dengue virus 1 to 4 antigen cocktail were tested in the two commercial assays. The dipstick ELISA missed 7 of 94 positive samples, for a sensitivity of 92.6%, while the immunochromatographic card assay missed two positive samples, for a sensitivity of 97.9%. Of the 70 negative samples, four were false positive by the dipstick ELISA and two were false positive in the immunochromatographic card assay, resulting in specificities of 94.3 and 97.1%, respectively. Both commercial assays provide sensitive and specific detection of anti-dengue virus IgM antibody and could prove useful in settings where the microplate ELISA is impractical.

* Corresponding author. Mailing address: Viral and Rickettsial Diseases Department, Code 41, Naval Medical Research Center, 8901 Wisconsin Ave., Bethesda, MD 20889-5607. Phone: (301) 319-7442. Fax: (301) 319-7460. E-mail: WuS{at}nmripo.nmri.nnmc.navy.mil.

Clinical and Diagnostic Laboratory Immunology, January 2000, p. 106-110, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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