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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 114-118, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of Specific Immune Responses of Mice Inoculated with Recombinant Vaccinia Virus Expressing an 18-Kilodalton Outer Membrane Protein of Brucella abortus

Ramesh Vemulapalli,1,* Silvio Cravero,1,2 Christine L. Calvert,1 Thomas E. Toth,1 Nammalwar Sriranganathan,1 Stephen M. Boyle,1 Osvaldo L. Rossetti,2 and Gerhardt G. Schurig1

Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Instituo Nacional de Tecnologia Agropecuaria, Provincia Buenos Aires, Argentina2

Received 16 August 1999/Returned for modification 27 September 1999/Accepted 4 November 1999

Using the shuttle vector pMCO2 and the vaccinia virus wild-type WR strain, we constructed a recombinant virus expressing an 18-kDa outer membrane protein of Brucella abortus. BALB/c mice inoculated with this virus produced 18-kDa protein-specific antibodies, mostly of immunoglobulin G2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein-18-kDa protein fusion resulted in lymphocyte proliferation and gamma interferon production. However, these mice were not protected against a challenge with the virulent strain B. abortus 2308. Disruption of the 18-kDa protein's gene in vaccine strain B. abortus RB51 did not affect either the strain's protective capabilities or its in vivo attenuation characteristics. These observations suggest that the 18-kDa protein plays no role in protective immunity.

* Corresponding author. Mailing address: Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1410 Prices Fork Rd., Blacksburg, VA 24061. Phone: (540) 231-7757. Fax: (540) 231-3426. E-mail: rvemulap{at}vt.edu.

Clinical and Diagnostic Laboratory Immunology, January 2000, p. 114-118, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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