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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 45-48, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Optimization of the Weck-Cel Collection Method for Quantitation of Cytokines in Mucosal Secretions

Lisa Cencia Rohan, Robert P. Edwards, Lori A. Kelly, Kelly A. Colenello, Frederick P. Bowman,dagger and Peggy A. Crowley-Nowick*

Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania

Received 24 May 1999/Returned for modification 12 July 1999/Accepted 20 September 1999

Measurement of immune components in mucosal secretions is important for the evaluation of local immunity at the mucosal surfaces. The Weck-Cel ophthalmic sponge provides a method for the collection of these secretions. The sponge absorbs a relatively large volume of material, therefore allowing for quantitation of multiple immune components. Additionally, it provides a method in which the same device may be used to collect specimens from different mucosal sites, such as the genital tract and oral cavity. This sampling technique has successfully been applied for collection and measurement of antibody in oral and genital tract secretions. The purpose of this work was to optimize the extraction of protein from the sponge matrix. Of particular interest was the recovery of cytokines from the sponge. Satisfactory recovery of the cytokines interleukin 1beta (IL-1beta ), IL-2, IL-5, IL-12, IL-6, IL-8, IL-10, and granulocyte-macrophage colony-stimulating factor was obtained. However, IL-4 and gamma interferon recovery rates remained low. Using an alteration of the published extraction method, cytokine concentrations were measured in cervical secretions from women using oral contraceptives. The data revealed detectable concentrations of IL-6, IL-10, IL-8, and IL-12 on cycle days 9 and 20. The proposed technique provides an easy, practical, and consistent method for collection of nonconventional body fluids, such as cervicovaginal fluids and saliva, for the assay of immunoglobulins and several cytokines.

* Corresponding author. Present address: Brigham and Women's Hospital, Harvard Medical School, 75 Francis St., Thorn 231, Boston, MA 02115. Phone: (617) 278-0464. Fax: (617) 264-5161. E-mail: pegc{at}earthlink.net.

dagger Present address: Department of Obstetrics, Gynecology and Reproductive Biology, Fearing Research Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.

Clinical and Diagnostic Laboratory Immunology, January 2000, p. 45-48, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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