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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 301-306, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Clustering of Clinical Strains of Helicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis

Helena Enroth,1 Thomas Åkerlund,2 Anna Sillén,2 and Lars Engstrand1,2,*

Department of Medical Epidemiology, Karolinska Institute,1 and Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden

Received 22 March 1999/Returned for modification 21 June 1999/Accepted 22 November 1999

Strain variations of Helicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether H. pylori expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the H. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that H. pylori exhibits.

* Corresponding author. Mailing address: Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden. Phone: (46) 8 457 24 15. Fax: (46) 8 30 17 97. E-mail: lars.engstrand{at}smi.ki.se.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 301-306, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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