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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 314-317, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads

Bruno Guy,* Catherine Testart, Sophie Gimenez, Violette Sanchez, Philippe Lheritier, Danielle Rossin, Michèle Mignon, Bernard Danve, and Emanuelle Trannoy

Research Department, Aventis Pasteur, Campus Merieux, 69280 Marcy L'Etoile, France

Received 3 September 1999/Returned for modification 9 November 1999/Accepted 8 December 1999

Polymorphonuclear cells (PMNs) from healthy donors and differentiated HL-60 cells were compared in an opsonophagocytic assay using fluorescent latex beads coated with Streptococcus pneumoniae polysaccharide conjugates. Serum-specific phagocytosis was efficiently mediated by both sources of cells, as measured by flow cytometry, but the mean number of beads ingested per cell was three- to fivefold higher when PMNs were used than when HL-60 cells were used. Nevertheless, differentiated HL-60 cells could be a convenient and standardized source of cells to evaluate the functionality of specific antibodies to vaccine candidates as a coating on fluorescent beads.

* Corresponding author. Mailing address: Research Department, Aventis Pasteur, Campus Merieux, 1541 Av. Marcel Merieux, 69280 Marcy L'Etoile, France. Phone: 33 (0)4 37 37 38 75. Fax: 33 (0)4 37 37 36 39. E-mail: Bruno.Guy{at}aventis.com.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 314-317, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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