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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 318-321, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Potentially Diagnostic Leishmania braziliensis Antigens in Human Cutaneous Leishmaniasis by Immunoblot Analysis

Maria Edileuza F. Brito,1 Mitzi G. Mendonça,2 Yara M. Gomes,1 Márcio L. Jardim,2 and Frederico G. C. Abath1,*

Departmento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz,1 and Departmento de Dermatologia, Universidade Federal de Pernambuco,2 Cidade Universitária, 50670-420 Recife, Brazil

Received 21 July 1999/Returned for modification 9 November 1999/Accepted 29 December 1999

The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens of Leishmania braziliensis. The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.

* Corresponding author. Mailing address: Centro de Pesquisas Aggeu Magalhães, Av. Prof. Moraes Rego s/n, Cidade Universitária, 50670-420 Recife, Brazil. Phone: 55-81-2714000. Fax: 55-81-4531911. E-mail: fabath{at}gene.dbbm.fiocruz.br.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 318-321, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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