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Clinical and Diagnostic Laboratory Immunology, May 2000, p. 451-456, Vol. 7, No. 3
Department of Virology, National Institute of
Public Health, Oslo,1 Department of
Microbiology, Sogn and Fjordane Central Hospital,
Førde,2 Institute of Microbiology, The
National Hospital, Oslo,3 Department of
Microbiology, Nordland Central Hospital, Bodø,4
Department of Microbiology, Rogaland Central Hospital,
Stavanger,5 Laboratory of
Microbiology, Vestfold Central Hospital,
Tønsberg,6 Department of
Microbiology, Ullevål University Hospital,
Oslo,7 Department of Microbiology,
Vest-Agder Central Hospital, Kristiansand,8
Department of Microbiology, Akershus Central Hospital,
Nordbyhagen,9 and Department of
Microbiology and Immunology, Haukeland University Hospital,
Bergen,10 Norway
Received 25 October 1999/Returned for modification 5 January
2000/Accepted 25 February 2000
Ten microbiological departments in Norway have participated in a
multicenter evaluation of the following commercial tests for detection
of Epstein-Barr virus (EBV)-specific and heterophile antibodies: CAPTIA
Select viral capsid antigen (VCA)-M/G/EBNA (Centocor Inc.), Enzygnost
anti-EBV/immunoglobulin M (IgM) and IgG (Dade Behring), Vironostika EBV
VCA IgM/IgG/EBNA enzyme-linked immunosorbent assay (ELISA) (Organon
Teknika), SEROFLUOR immunofluorescence assay and EBV Combi-Test
(Institute Virion Ltd.), anti-EBV recombinant IgM- and IgG-early
antigen/EBNA IgG ELISA (Biotest Diagnostics), EBV IgM/IgG/EBNA ELISA
(Gull Laboratories), Paul-Bunnell-Davidsohn test (Sanofi Diagnostics
Pasteur), Monosticon Dri-Dot (Organon Teknika), Avitex-IM (Omega
Diagnostics Ltd.), Alexon Serascan infectious mononucleosis test
(Alexon Biomedical Inc.), Clearview IM (Unipath Ltd.), and Cards±OS
Mono (Pacific Biotech, Inc.). The test panel included sera from
patients with primary EBV infection, immunocompromised patients with
recent cytomegalovirus infection, healthy persons (blood donors), and
EBV-seronegative persons. Among the tests for EBV-specific antibodies
the sensitivity was good, with only small differences between the
different assays. However, there was a greater variation in
specificity, which varied between 100% (Enzygnost) and 86% (Biotest).
Tests for detection of heterophile antibodies based on purified or
selected antigen (Avitex, Alexon, Clearview IM, and Cards±OS Mono)
were more sensitive than the Paul-Bunnell-Davidsohn and Monosticon tests.
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of 12 Commercial Tests for Detection of
Epstein-Barr Virus-Specific and Heterophile Antibodies
*
Corresponding author. Mailing address: Department of
Virology, National Institute of Public Health, Postbox 4404 Torshov, 0403 Oslo, Norway. Phone: 47 22 04 23 12. Fax: 47 22 04 24 47. E-mail:
anne-lise.bruu{at}folkehelsa.no.
Clinical and Diagnostic Laboratory Immunology, May 2000, p. 451-456, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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