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Clinical and Diagnostic Laboratory Immunology, May 2000, p. 504-506, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diagnosis of Enterocytozoon bieneusi by PCR in Stool Samples Eluted from Filter Paper Disks

Silvana Carnevale,* Jorge N. Velásquez, Jorge H. Labbé, Agustín Chertcoff, Marta G. Cabrera, and Mónica I. Rodríguez

Departamento de Parasitología, Instituto Nacional de Microbiología "Dr. Carlos G. Malbrán," and Hospital Municipal de Infecciosas "Dr. Francisco J. Muñiz," Buenos Aires, Argentina

Received 2 November 1999/Returned for modification 7 January 2000/Accepted 3 February 2000

We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.

* Corresponding author. Mailing address: Lituania 520, (1826) Remedios de Escalada, Buenos Aires, Argentina. Phone: 54 11 4242 0883. Fax: 54 11 4301 7437. E-mail: jorsil{at}overnet.com.ar.

Clinical and Diagnostic Laboratory Immunology, May 2000, p. 504-506, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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