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 Previous Article

Clinical and Diagnostic Laboratory Immunology, May 2000, p. 519-521, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Monoclonal Antibody Differentiation of Mycoplasma mycoides subsp. mycoides Small-Colony Strains Causing Contagious Bovine Pleuropneumonia from Less Important Large-Colony Strains

Fred R. Rurangirwa,1,* Patrick S. Shompole,1 Anderson N. Wambugu,2 and Travis C. McGuire1

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,1 and Biotechnology and Immunology Laboratory, Kenya Agricultural Research Institute, Kabete, Kenya2

Received 4 November 1999/Returned for modification 29 December 1999/Accepted 10 March 2000

Monoclonal antibody (MAb) PK-2 inhibited the in vitro growth of nine Mycoplasma mycoides subsp. mycoides small-colony strains. In contrast to the results with polyclonal antisera, growth inhibition by MAb PK-2 was specific for M. mycoides subsp. mycoides small-colony strains and constituted a reliable means of distinguishing them from other mycoplasmas.

* Corresponding author. Mailing address: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040. Phone: (509) 335-6023. Fax: (509) 335-8529. E-mail: ruvuna{at}vetmed.wsu.edu.

Clinical and Diagnostic Laboratory Immunology, May 2000, p. 519-521, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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