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Clinical and Diagnostic Laboratory Immunology, July 2000, p. 714-716, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Viability and Functional Activity of Cryopreserved Mononuclear Cells

Adriana Weinberg,1,* Li Zhang,1 Darby Brown,1 Alejo Erice,2 Bruce Polsky,3 Martin S. Hirsch,4 Susan Owens,5 and Karan Lamb6

University of Colorado Health Sciences Center, Denver, Colorado1; University of Minnesota, Minneapolis, Minnesota2; St. Luke's-Roosevelt Hospital Center, New York, New York3; Massachusetts General Hospital, Harvard School of Public Health, Boston, Massachusetts4; and Frontier Science & Technology Research Foundation5 and Adult AIDS Clinical Trials Group Operations Center,6 Washington, D.C.

Received 18 January 2000/Returned for modification 21 March 2000/Accepted 11 May 2000

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

* Corresponding author. Mailing address: University of Colorado Health Sciences Center, Campus box C227, 4200 East Ninth Ave., Denver, CO 80262. Phone: (303) 315-4624. Fax: (303) 315-6955. E-mail: adriana.weinberg{at}uchsc.edu.

Clinical and Diagnostic Laboratory Immunology, July 2000, p. 714-716, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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Copyright © 2000 by the American Society for Microbiology. All rights reserved.