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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 865-866, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of a Chemiluminescent Immunoassay with Two Microparticle Enzyme Immunoassays for Detection of Hepatitis B Virus Surface Antigen

Robert J. A. Diepersloot,1,* Yvonne van Zantvliet-van Oostrom,1 and Curt A. Gleaves2

Laboratory for Medical Microbiology, Diakonessen Hospital, Utrecht, The Netherlands,1 and Infectious Disease and Molecular Diagnostics, Providence Portland Medical Center, Portland, Oregon2

Received 11 February 2000/Returned for modification 31 May 2000/Accepted 1 August 2000

A comparative evaluation of the following commercial immunoassays for the detection of hepatitis B virus surface antigen (HBsAg) was performed: the Abbott AxSYM, Abbott IMx, and DPC IMMULITE assays. The specificity was 100% for all assays. Twelve samples were identified and were confirmed to be positive for HBsAg by all three methods. One additional sample was identified as reactive and was confirmed to be positive by the Abbott AxSYM assay only. Prior to confirmation testing the DPC IMMULITE assay produced significantly fewer false-positive results than the Abbott AxSYM assay (P < 0.05).

* Corresponding author. Mailing address: Laboratory for Medical Microbiology, Diakonessen Hospital, 1, Bosboomstraat, 3582 KE Utrecht, The Netherlands. Phone: 31 30 256 65 66. Fax: 31 30 256 66 95. E-mail: rdiepersloot{at}diakhuis.nl

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 865-866, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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