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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 980-982, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inhibition of Human Peripheral Blood Neutrophil Respiratory Burst by Alcohol-Based Venipuncture Site Disinfection

Norbert Reinisch,1 Christian J. Wiedermann,2 and Giovanni Ricevuti1,*

Section of Internal Medicine and Nephrology, Department of Internal Medicine and Therapeutics, University of Pavia, I-27100 Pavia, Italy,1 and Division of General Internal Medicine, Department of Internal Medicine, University of Innsbruck, A-6020 Innsbruck, Austria2

Received 23 February 2000/Returned for modification 31 May 2000/Accepted 27 July 2000

Ethanol inhibits the respiratory burst of neutrophils. Therefore, the effects of alcohol-based skin disinfection on oxygen metabolism in neutrophils were tested using 70% ethanol or an ethanol-isopropanol-n-propanol mixture. Neutrophil respiratory burst activity as assessed fluorometrically by oxidation of 2',7'-dichlorofluorescein diacetate increased at 10 min after disinfection with 70% ethanol compared to the activity at 30 s. The increase was significant for triggering oxidative burst with formylpeptide but not with phorbol myristate acetate.

* Corresponding author. Mailing address: IRCCS Ospedale S. Matteo, Piazzale Golgi 2, I-27100 Pavia, Italy. Phone: 39-0382-502592. Fax. 39-0382-526950. E-mail: g.ricevuti{at}smatteo.pv.it.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 980-982, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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