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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 987-989, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Confirmation of Human Immunodeficiency Virus (HIV) Type 2 in HIV-Seropositive Subjects in South India

R. Kannangai,1 S. Ramalingam,1 K. J. Prakash,1 O. C. Abraham,2 R. George,3 R. C. Castillo,4 D. H. Schwartz,4 M. V. Jesudason,5 and G. Sridharan1,*

Departments of Clinical Virology,1 Internal Medicine Unit I,2 Dermatology,3 and Clinical Microbiology,5 Christian Medical College Hospital, Vellore, India, 632004, and Department of Molecular Microbiology and Immunology, The Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 212054

Received 4 February 2000/Returned for modification 24 April 2000/Accepted 6 September 2000

Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.

* Corresponding author. Mailing address: Department of Clinical Virology, Christian Medical College Hospital, Vellore 632004, India. Phone: 91 (416) 222102. Fax: 91 (416) 232035. E-mail: gsridhar{at}viro.cmc.ernet.in or g_sridharan_in{at}yahoo.com.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 987-989, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vijayakumar, T.-S., David, S., Selvaraj, K., Viswanathan, T., Kannangai, R., Sridharan, G. (2005). Performance of a Rapid Immunochromatographic Screening Test for Detection of Antibodies to Human Immunodeficiency Virus Type 1 (HIV-1) and HIV-2: Experience at a Tertiary Care Hospital in South India. J. Clin. Microbiol. 43: 4194-4196 [Abstract] [Full Text]  
  • Leano, P. S., Kageyama, S., Espantaleon, A., Maniar, J., Iwasaki, M., Saple, D., Yoshihara, N., Kurimura, T., Agdamag, D. M. (2003). Introduction of Human Immunodeficiency Virus Type 2 Infection in the Philippines. J. Clin. Microbiol. 41: 516-518 [Abstract] [Full Text]  


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