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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 143-149, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.143-149.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The AIDA Autotransporter System Is Associated with F18 and Stx2e in Escherichia coli Isolates from Pigs Diagnosed with Edema Disease and Postweaning Diarrhea

Ulla Niewerth,1 Andreas Frey,1 Thomas Voss,1 Chantal Le Bouguénec,2 Georg Baljer,3 Sylvia Franke,3 and M. Alexander Schmidt1,*

Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität, Münster,1 Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität, Giessen,3 Germany, and Unité de Pathogénie Bacteriénne des Muqueuses, Institut Pasteur, Paris, France 2

Received 11 April 2000/Returned for modification 28 August 2000/Accepted 25 September 2000

Pathogenic Escherichia coli strains are known to cause edema disease (ED) and postweaning diarrhea (PWD) in piglets. Although the exact mechanisms of pathogenicity that lead to ED-PWD remain to be elucidated, E. coli-borne Shiga-like toxin and adhesion-mediating virulence factors such as F18 adhesin or F4 fimbriae are believed to play a central role in ED-PWD. In light of these observations we investigated whether another E. coli adhesin, the plasmid-encoded AIDA (adhesin involved in diffuse adherence) might also be present in ED-PWD-causing E. coli isolates. For rapid screening for the AIDA system in large numbers of isolates, a multiplex PCR method along with a duplex Western blot procedure was developed. When screening 104 strains obtained from pigs with or without ED-PWD, we observed a high prevalence of the AIDA operon in porcine E. coli isolates, with over 25% of all strains being AIDA positive, and we could demonstrate a significant association of the intact AIDA gene (orfB) with ED-PWD, while defects in orfB were associated with the absence of disease. Although our data hint toward a contribution of AIDA to ED-PWD, further studies will be necessary since the presence of the AIDA genes was also associated with the presence of the Shiga-like toxin and F18 adhesin genes, two reported virulence factors for ED-PWD.

* Corresponding author. Mailing address: Institut für Infektiologie, ZMBE, Von-Esmarch-Str. 56, D-48149 Münster, Germany. Phone: 49-251-8356469. Fax: 49-251-8356467. E-mail:infekt{at}uni-muenster.de.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 143-149, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.143-149.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sonntag, A.-K., Bielaszewska, M., Mellmann, A., Dierksen, N., Schierack, P., Wieler, L. H., Schmidt, M. A., Karch, H. (2005). Shiga Toxin 2e-Producing Escherichia coli Isolates from Humans and Pigs Differ in Their Virulence Profiles and Interactions with Intestinal Epithelial Cells. Appl. Environ. Microbiol. 71: 8855-8863 [Abstract] [Full Text]  
  • Muller, D., Benz, I., Tapadar, D., Buddenborg, C., Greune, L., Schmidt, M. A. (2005). Arrangement of the Translocator of the Autotransporter Adhesin Involved in Diffuse Adherence on the Bacterial Surface. Infect. Immun. 73: 3851-3859 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
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