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Clinical and Diagnostic Laboratory Immunology, March 2001, p. 241-244, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.241-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of the 16S rRNA Gene Sequence of Anaplasma centrale and Its Phylogenetic Relatedness to Other Ehrlichiae

Hisashi Inokuma,1,2 Yutaka Terada,3 Tugihiko Kamio,3 Didier Raoult,2 and Philippe Brouqui2,*

Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515,1 and National Institute of Animal Health, Tsukuba 305-0856,3 Japan, and Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille, France2

Received 31 August 2000/Returned for modification 19 October 2000/Accepted 20 November 2000

The nucleotide sequence of the Anaplasma centrale 16S rRNA gene was determined and compared with the sequences of ehrlichial bacteria. The sequence of A. centrale was closely related to Anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. A species-specific PCR was developed based upon the alignment data. The PCR can detect A. centrale DNA extracted from 10 infected bovine red blood cells in a reaction mixture. A. centrale DNA was amplified in the reaction, but not other related ehrlichial species.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 bd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33)-4-91-32-43-75. Fax: (33)-4-91-83-03-90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 241-244, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.241-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Inokuma, H., Beppu, T., Okuda, M., Shimada, Y., Sakata, Y. (2004). Detection of Ehrlichial DNA in Haemaphysalis Ticks Recovered from Dogs in Japan That Is Closely Related to a Novel Ehrlichia sp. Found in Cattle Ticks from Tibet, Thailand, and Africa. J. Clin. Microbiol. 42: 1353-1355 [Abstract] [Full Text]  
  • Shkap, V., Molad, T., Brayton, K. A., Brown, W. C., Palmer, G. H. (2002). Expression of Major Surface Protein 2 Variants with Conserved T-Cell Epitopes in Anaplasma centrale Vaccinates. Infect. Immun. 70: 642-648 [Abstract] [Full Text]  
  • Inokuma, H., Brouqui, P., Drancourt, M., Raoult, D. (2001). Citrate Synthase Gene Sequence: a New Tool for Phylogenetic Analysis and Identification of Ehrlichia. J. Clin. Microbiol. 39: 3031-3039 [Abstract] [Full Text]  


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