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Clinical and Diagnostic Laboratory Immunology, March 2001, p. 441-445, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.441-445.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Human T-Cell Responses to the Glucosyltransferases of Streptococcus mutans

Jean-San Chia,1,* Chiou-Mien You,1 Chung-Yi Hu,2 Bor-Luen Chiang,3 and Jen-Yang Chen1

Graduate Institute of Microbiology,1 School/Institute of Medical Technology,2 and Graduate Institute of Clinical Medicine,3 College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China

Received 21 July 2000/Returned for modification 29 September 2000/Accepted 15 November 2000

We previously reported differential humoral responses to glucosyltransferases (GTFs), with significantly higher saliva and serum antibody levels to GtfD than to GtfB or GtfC. To test the hypothesis that cellular immune responses to these molecules also may differ, peripheral blood mononuclear cell (PBMC) and T-cell proliferative responses in young adults and children with distinct genetic backgrounds were determined using purified recombinant GtfC and GtfD. PBMCs from all of the volunteers responded to GtfC and -D, but responses were directed predominantly towards GtfD and were major histocompatibility class II antigen dependent. A predominant T-cell response to GtfD, over GtfC, was detectable at various antigen concentrations ranging from 1 to 20 µg/ml and correlated with the differential serum immunoglobulin G (IgG) and salivary IgA antibody responses to the GTFs. Therefore, in naturally sensitized humans, Streptococcus mutans GTFs stimulate differential humoral and cellular immune responses, with the secreted form of GtfD eliciting a stronger response than the cell wall-associated form of GtfC.

* Corresponding author. Mailing address: No. 1, Jen Ai Rd., 1st Section, Room 713, Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan. Phone: 886-2-23970800, ext. 8222. Fax: 886-2-23915293. E-mail: chiajs{at}ha.mc.ntu.edu.tw.

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 441-445, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.441-445.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shun, C.-T., Lu, S.-Y., Yeh, C.-Y., Chiang, C.-P., Chia, J.-S., Chen, J.-Y. (2005). Glucosyltransferases of Viridans Streptococci Are Modulins of Interleukin-6 Induction in Infective Endocarditis. Infect. Immun. 73: 3261-3270 [Abstract] [Full Text]  
  • Chia, J.-S., Lien, H.-T., Hsueh, P.-R., Chen, P.-M., Sun, A., Chen, J.-Y. (2002). Induction of Cytokines by Glucosyltransferases of Streptococcus mutans. CVI 9: 892-897 [Abstract] [Full Text]  


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