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Clinical and Diagnostic Laboratory Immunology, May 2001, p. 499-502, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.499-502.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

Hideaki Ikejima,1 Shusaku Haranaga,1 Hiromu Takemura,1,dagger Tsutomu Kamo,2 Youichi Takahashi,2 Herman Friedman,1 and Yoshimasa Yamamoto1,*

Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, Florida 33612,1 and Department of Neurology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan2

Received 15 September 2000/Returned for modification 6 December 2000/Accepted 22 January 2001

Since current studies indicate the possible involvement of Chlamydia pneumoniae in the pathogenesis of multiple sclerosis (MS), demonstration of C. pneumoniae in the cerebrospinal fluid (CSF) of patients with MS is highly desirable. However, there is controversy concerning the detection of C. pneumoniae in CSFs from MS patients due to the lack of a standard protocol for extraction and detection of C. pneumoniae DNA. In this regard, we attempted to establish a highly effective extraction protocol for C. pneumoniae DNA from CSFs utilizing a commercial kit and a PCR detection method. The extraction and PCR detection protocol established in this study succeeded in detecting as few as 20 C. pneumoniae organisms in 200 µl of mock CSF. The use of this protocol to detect C. pneumoniae DNA in CSFs revealed that 68% of CSF samples obtained from patients with MS were positive (11 out of 16 samples) for chlamydia DNA. Thus, the protocol established here is sensitive enough to detect chlamydia DNA from CSFs and can be used by other laboratories for evaluation of the presence of chlamydiae in CSFs because the protocol is based on the use of a commercial kit.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, 12901 Bruce B. Downs Blvd., Tampa, FL 33612. Phone: (813) 974-2332. Fax: (813) 974-4151. E-mail: yyamamot{at}hsc.usf.edu.

dagger Present address: Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 499-502, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.499-502.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sriram, S., Yao, S.-y., Stratton, C., Calabresi, P., Mitchell, W., Ikejima, H., Yamamoto, Y. (2002). Comparative Study of the Presence of Chlamydia pneumoniae in Cerebrospinal Fluid of Patients with Clinically Definite and Monosymptomatic Multiple Sclerosis. CVI 9: 1332-1337 [Abstract] [Full Text]  
  • Yamamoto, Y. (2002). PCR in Diagnosis of Infection: Detection of Bacteria in Cerebrospinal Fluids. CVI 9: 508-514 [Full Text]  


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