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Clinical and Diagnostic Laboratory Immunology, May 2001, p. 628-631, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.628-631.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Gamma Interferon Expression in CD8+ T Cells Is a Marker for Circulating Cytotoxic T Lymphocytes That Recognize an HLA A2-Restricted Epitope of Human Cytomegalovirus Phosphoprotein pp65

Smita A. Ghanekar,1,* Laurel E. Nomura,1 Maria A. Suni,1 Louis J. Picker,2 Holden T. Maecker,1 and Vernon C. Maino1

BD Biosciences, Immunocytometry Systems, San Jose, California 95131,1 and Vaccine and Gene Therapy Institute, Oregon Health Sciences University, Portland, Oregon 972012

Received 16 November 2000/Returned for modification 2 January 2001/Accepted 1 February 2001

Antigen-specific CD8+ T cells with cytotoxic activity are often critical in immune responses to infectious pathogens. To determine whether gamma interferon (IFN-gamma ) expression is a surrogate marker for cytotoxic T lymphocytes (CTL), human cytomegalovirus-specific CTL responses were correlated with CD8+ T-cell IFN-gamma expression determined by cytokine flow cytometry. A strong positive correlation was observed between specific lysis of peptide-pulsed targets in a 51Cr release assay and frequencies of peptide-activated CD8+ T cells expressing IFN-gamma at 6 h (r2 = 0.72) or 7 days (r2 = 0.91). Enumeration of responding cells expressing perforin, another marker associated with CTL, did not improve this correlation. These results demonstrate that IFN-gamma expression can be a functional surrogate for identification of CTL precursor cells.

* Corresponding author. Mailing address: BD Biosciences, 2350 Qume Dr., San Jose, CA 95131. Phone: (408) 954-4177. Fax: (408) 954-2156. E-mail: smita_ghanekar{at}bdis.com.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 628-631, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.628-631.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.