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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 695-701, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.695-701.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Induction by a Lactic Acid Bacterium of a Population of CD4+ T Cells with Low Proliferative Capacity That Produce Transforming Growth Factor beta  and Interleukin-10

Thierry von der Weid,* Christine Bulliard, and Eduardo J. Schiffrin

Nestec SA, Nestlé Research Center, 1000 Lausanne 26, Switzerland

Received 23 December 2000/Returned for modification 7 February 2001/Accepted 15 March 2001

We investigated whether certain strains of lactic acid bacteria (LAB) could antagonize specific T-helper functions in vitro and thus have the potential to prevent inflammatory intestinal immunopathologies. All strains tested induced various levels of both interleukin-12 (IL-12) and IL-10 in murine splenocytes. In particular, Lactobacillus paracasei (strain NCC2461) induced the highest levels of these cytokines. Since IL-12 and IL-10 have the potential to induce and suppress Th1 functions, respectively, we addressed the impact of this bacterium on the outcome of CD4+ T-cell differentiation. For this purpose, bacteria were added to mixed lymphocyte cultures where CD4+ T-cells from naive BALB/c mice were stimulated weekly in the presence of irradiated allogeneic splenocytes. In these cultures, L. paracasei NCC2461 strongly inhibited the proliferative activity of CD4+ T cells in a dose-dependent fashion. This was accompanied by a marked decrease of both Th1 and Th2 effector cytokines, including gamma interferon, IL-4, and IL-5. In contrast, IL-10 was maintained and transforming growth factor beta  (TGF-beta ) was markedly induced in a dose-dependent manner. The bacteria were not cytotoxic, because cell viability was not affected after two rounds of stimulation. Thus, unidentified bacterial components from L. paracasei NCC2461 induced the development of a population of CD4+ T cells with low proliferative capacity that produced TGF-beta and IL-10, reminiscent of previously described subsets of regulatory cells implicated in oral tolerance and gut homeostasis.

* Corresponding author. Mailing address: Nestec SA, Nestlé Research Center, P.O. Box 44, 1000 Lausanne 26, Switzerland. Phone: 41 21 785 89 54. Fax: 41 21 785 89 25. E-mail: thierry.von-der-weid{at}rdls.nestle.com.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 695-701, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.695-701.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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