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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 825-827, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.825-827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Generation and Serological Characterization of Murine Monoclonal Antibodies against O Antigens from Acinetobacter Reference Strains

Ralph Pantophlet,dagger Lore Brade, and Helmut Brade*

Research Center Borstel, Division of Medical and Biochemical Microbiology, Borstel, Germany

Received 27 December 2000/Returned for modification 12 March 2001/Accepted 20 April 2001

O-antigen-specific monoclonal antibodies were generated against Acinetobacter strains from international type culture collections and characterized by enzyme immunoassay and Western and colony blotting. The antibodies aid in the further completion of an O-serotyping scheme for Acinetobacter and, due to their high specificity, are especially useful to all working with these strains.

* Corresponding author. Mailing address: Research Center Borstel, Center for Medicine and Biosciences, Division of Medical and Biochemical Microbiology, Parkallee 22, 23845 Borstel, Germany. Phone: 49-4537-188-474. Fax: 49-4537-188-419. E-mail: hbrade{at}fz-borstel.de.

dagger Present address: The Scripps Research Institute, Department of Immunology, La Jolla, Calif.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 825-827, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.825-827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.