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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 832-836, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.832-836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

groEL Encodes a Highly Antigenic Protein in Burkholderia pseudomallei

Patrick C. Y. Woo,1 Patricia K. L. Leung,1 Samson S. Y. Wong,1 Pak-Leung Ho,1 and Kwok-Yung Yuen1,2,*

Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and HKU-Pasteur Research Centre,2 Hong Kong, China

Received 26 October 2000/Returned for modification 12 March 2001/Accepted 24 April 2001

No recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of the groEL gene, which encodes an immunogenic protein of Burkholderia pseudomallei. Bidirectional DNA sequencing of groEL revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by groEL is homologous to the chaperonins encoded by the groEL genes of other bacteria. It has 98% amino acid identity with the GroEL of Burkholderia cepacia, 98% amino acid identity with the GroEL of Burkholderia vietnamiensis, and 82% amino acid identity with the GroEL of Bordetella pertussis. Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong, China. Phone: (852) 28554892. Fax: (852) 28551241. E-mail: hkumicro{at}hkucc.hku.hk.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 832-836, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.832-836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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