This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liang, Z.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liang, Z.
Right arrow Articles by Raoult, D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 847-849, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.847-849.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Production of Bartonella Genus-Specific Monoclonal Antibodies

Zhongxing Liang, Bernard La Scola, Hubert Lepidi, and Didier Raoult*

Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Mediterranée, 13385 Marseille Cédex, France

Received 26 October 2000/Returned for modification 22 January 2001/Accepted 3 April 2001

Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 different Bartonella species were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted with Chlamydia psittaci and Chlamydia trachomatis at low titers. The identification of a common Bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it in Escherichia coli. Moreover, these MAbs allow all Bartonella species to be identified to the genus level.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 Blvd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33) 4 91 32 43 75. Fax: (33) 4 91 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 847-849, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.847-849.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Foucault, C., Rolain, J. M., Raoult, D., Brouqui, P. (2004). Detection of Bartonella quintana by Direct Immunofluorescence Examination of Blood Smears of a Patient with Acute Trench Fever. J. Clin. Microbiol. 42: 4904-4906 [Abstract] [Full Text]  
  • Gilmore, R. D. Jr., Carpio, A. M., Kosoy, M. Y., Gage, K. L. (2003). Molecular Characterization of the sucB Gene Encoding the Immunogenic Dihydrolipoamide Succinyltransferase Protein of Bartonella vinsonii subsp. berkhoffii and Bartonella quintana. Infect. Immun. 71: 4818-4822 [Abstract] [Full Text]  
  • Iredell, J., McHattan, J., Kyme, P., Dillon, B., Blanckenberg, D. (2002). Antigenic and Genotypic Relationships between Bartonella henselae Strains. J. Clin. Microbiol. 40: 4397-4398 [Full Text]  
  • Yamamoto, K., Chomel, B. B., Kasten, R. W., Hew, C. M., Weber, D. K., Lee, W. I., Droz, S., Koehler, J. E. (2002). Experimental Infection of Domestic Cats with Bartonella koehlerae and Comparison of Protein and DNA Profiles with Those of Other Bartonella Species Infecting Felines. J. Clin. Microbiol. 40: 466-474 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»