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Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1018-1020, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1018-1020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Determination of Human Immunodeficiency Virus Type 1 Subtypes by a Rapid Method Useful for the Routine Diagnostic Laboratory

Harald H. Kessler,* Doris Deuretzbacher, Evelyn Stelzl, Elisabeth Daghofer, Brigitte I. Santner, and Egon Marth

Molecular Diagnostics Laboratory, Institute of Hygiene, Karl-Franzens-University, A-8010 Graz, Austria

Received 4 January 2001/Returned for modification 14 March 2001/Accepted 7 June 2001

The existence of human immunodeficiency virus type 1 (HIV-1) subtypes has many important implications for the global evolution of HIV and for the evaluation of pathogenicity, transmissibility, and candidate HIV vaccines. The aim of this study was to establish a rapid method for determination of HIV-1 subtypes useful for a routine diagnostic laboratory and to investigate the distribution of HIV-1 subtypes in Austrian patients. Samples were tested by a subtyping method based on a 1.3-kb sequence of the polymerase gene generated by a commercially available drug resistance assay. The generated sequence was subtyped by means of an HIV sequence database. Results of 74 routine samples revealed subtype B (71.6%) as the predominant subtype, followed by subtype A (13.5%) and subtype C (6.8%). Subtypes E, F, G, and AE (CM240) were also detected. This subtyping method was found to be very easy to handle, rapid, and inexpensive and has proved suitable for high-throughput routine diagnostic laboratories. The specific polymerase gene sequence, however, must be existent.

* Corresponding author. Mailing address: Molecular Diagnostics Laboratory, Institute of Hygiene, Karl-Franzens-University Graz, Universitaetsplatz 4, A-8010 Graz, Austria. Phone: 43-316-380-4363. Fax: 43-316-380-9649. E-mail: harald.kessler{at}uni-graz.at.

Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1018-1020, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1018-1020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muller, Z., Stelzl, E., Bozic, M., Haas, J., Marth, E., Kessler, H. H. (2004). Evaluation of Automated Sample Preparation and Quantitative PCR LCx Assay for Determination of Human Immunodeficiency Virus Type 1 RNA. J. Clin. Microbiol. 42: 1439-1443 [Abstract] [Full Text]  


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