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Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1031-1035, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1031-1035.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Reactivity of MEST-1 (Antigalactofuranose) with Trypanosoma cruzi Glycosylinositol Phosphorylceramides (GIPCs): Immunolocalization of GIPCs in Acidic Vesicles of Epimastigotes

Erika Suzuki,1 Renato A. Mortara,2 Helio K. Takahashi,1 and Anita H. Straus1,*

Departamento de Bioquímica1 and Departamento de Microbiologia, Imunologia e Parasitologia,2 Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, SP 04023-900, Brazil

Received 23 April 2001/Accepted 11 July 2001

Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.

* Corresponding author. Mailing address: Departamento de Bioquímica, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP 04023-900, Brazil. Phone and fax: 55-11-5579-2509. E-mail: straus.bioq{at}epm.br.

Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1031-1035, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1031-1035.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.