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Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1150-1155, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1150-1155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of Recombinant Envelope Proteins for Serological Diagnosis of Dengue Virus Infection in an Immunochromatographic Assay

Andrea J. Cuzzubbo,1,* Timothy P. Endy,2 Ananda Nisalak,2 Siripen Kalayanarooj,3 David W. Vaughn,4 Steven A. Ogata,5 David E. Clements,5 and Peter L. Devine1,dagger

PanBio Ltd., Windsor 4030, Queensland, Australia1; Department of Virology, Armed Forces Research Institute of Medical Sciences,2 and Queen Sirikit National Institute for Child Health,3 Bangkok, Thailand; Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland4; and Hawaii Biotechnology Group Inc., Aiea, Hawaii5

Received 18 June 2001/Returned for modification 23 July 2001/Accepted 22 August 2001

An immunochromatographic test that incorporates recombinant antigens (Dengue Duo Rapid Strip Test; PanBio, Brisbane, Australia) has recently become commercially available. This assay is performed in 15 min and detects both immunoglobulin M (IgM) and IgG in a capture format. The four recombinant proteins used represent the N-terminal 80% of the viral envelope glycoproteins of dengue viruses 1, 2, 3, and 4, respectively. The sensitivity and specificity of the recombinant-antigen-based assay were 90 and 86%, respectively. The similar diagnostic performance of these antigens to that of enzyme-linked immunosorbent assays using whole dengue virus suggests that they mimic whole dengue viruses in primary structure and epitope conformation. These results suggest that recombinant proteins can be used in diagnostic assays for dengue to overcome safety issues associated with the use of whole virus.

* Corresponding author. Mailing address: PanBio Ltd., 116 Lutwyche Rd., Windsor 4030, Queensland, Australia. Phone: 61-7-3357-1177. Fax: 61-7-3357-1222. E-mail: andrea_cuzzubbo{at}panbio.com.au.

dagger Present address: Progen Industries Ltd., Brisbane, Queensland, Australia. Phone: 61-7-3273-9176. Fax: 61-7-3375-9318. E-mail: peter.devine{at}progen.com.au.

Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1150-1155, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1150-1155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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