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Clinical and Diagnostic Laboratory Immunology, March 2002, p. 308-312, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.308-312.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immunoglobulin G, A, and M Responses in Serum and Circulating Immune Complexes Elicited by the 16-Kilodalton Antigen of Mycobacterium tuberculosis

Alamelu Raja,1* K. R. Uma Devi,1 B. Ramalingam,1 and Patrick J. Brennan2

Department of Immunology, Tuberculosis Research Centre (ICMR), Chetput, Chennai-600 031, India,1 Department of Microbiology, Colorado State University, Fort Collins, Colorado 805232

Received 12 March 2001/ Returned for modification 14 June 2001/ Accepted 13 November 2001

The 16-kDa cytosolic antigen of M. tuberculosis was purified to homogeneity by molecular sieving chromatography, and the diagnostic potential of the antigen was evaluated in various categories of patients by enzyme-linked immunosorbent assay (ELISA). The immunoglobulin G (IgG), IgA, and IgM antibody levels to 16-kDa antigen were estimated in the two polar groups, namely, smear- and culture-positive pulmonary tuberculosis (S+C+) patients and healthy subjects (HS). Sensitivities of 62, 52 and 11% with specificities of 100, 97, and 95% were obtained for the three isotypes, respectively. The total number of positives by a combination of the three isotypes was analyzed in the polar groups, and the sensitivity improved to 83% with a specificity of 93%. Even when a combination of IgG and IgA alone was considered, the sensitivity was 82% with a specificity of 97%. Polyethylene glycol precipitation of the circulating immune complex (CIC) in sera was carried out. The CIC-bound antibodies to 16-kDa antigen were assessed by ELISA in the S+C+, S-C+, and S-C- categories of patients. Measuring the IgG-IgA-IgM combination positivities of the CIC-bound antibodies gave sensitivities of 97.5, 100, and 45.3%, respectively. The specificity of the assay with these combinations was maintained at 95.4%.

* Corresponding author. Mailing address: Tuberculosis Research Centre (ICMR), Mayor V. R. Ramanathan Rd., Chetput, Chennai-600 031, India. Phone: 91 (044) 826 5425. Fax: 91 (044) 826 2137. E-mail: alameluraja{at}yahoo.com.

Clinical and Diagnostic Laboratory Immunology, March 2002, p. 308-312, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.308-312.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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