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Clinical and Diagnostic Laboratory Immunology, March 2002, p. 320-323, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.320-323.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cell-Associated Interleukin-8 in Cord Blood of Term and Preterm Infants

J. Dembinski,1* D. Behrendt,1 A. Heep,1 C. Dorn,2 J. Reinsberg,2 and P. Bartmann1

Department of Neonatalogy, Center of Pediatrics,1 Center of Obstetrics and Gynecology, University of Bonn, Bonn D-53113, Germany2

Received 10 July 2001/ Returned for modification 22 October 2001/ Accepted 26 November 2001

To assess the effect of gestational age and labor on the interleukin-8 (IL-8) concentration in whole cord blood and serum, IL-8 levels were determined simultaneously in cord blood serum and lysate in 134 infants. Following the elimination of some of the samples due to exclusion criteria, the data for 99 uninfected infants (71 term and 28 preterm) and 9 infants with neonatal bacterial infection delivered either vaginally or by elective or emergency cesarean section were analyzed. The effects of labor and gestational age were tested by analysis of variance. IL-8 was not detectable in the serum of 25 infants, whereas IL-8 levels in whole blood were measurable in all of the samples. The median IL-8 conncentrations in whole cord blood lysate were 106 pg/ml (range, 20 to 415 pg/ml) in preterm infants and 176 pg/ml (range, 34 to 1,667 pg/ml) in term infants. In contrast to the IL-8 levels in serum, IL-8 levels in whole blood were reduced after ECS. Gestational age had no independent effect on the IL-8 concentrations in either serum or whole blood; these concentrations increased in infected infants after labor. We conclude that the neonatal proinflammatory response to labor stress was more evident in the concentrations of IL-8 in whole blood than in serum. The levels of IL-8 in whole-blood lysate reflect proinflammatory stimulation in neonates and may be a useful diagnostic tool for the early diagnosis of neonatal infection.

* Corresponding author. Mailing address: Department of Neonatology, Center of Pediatrics, University of Bonn, Adenauerallee 119, D-53113 Bonn, Germany. Phone: 49-228-2873408. Fax: 49-228-2876291. E-mail: jdembinski{at}uni-bonn.de.

Clinical and Diagnostic Laboratory Immunology, March 2002, p. 320-323, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.320-323.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Behrendt, D, Dembinski, J, Heep, A, Bartmann, P (2004). Lipopolysaccharide binding protein in preterm infants. Arch. Dis. Child. Fetal Neonatal Ed. 89: F551-F554 [Abstract] [Full Text]  


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