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Clinical and Diagnostic Laboratory Immunology, March 2002, p. 477-481, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.477-481.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sodium Bicarbonate Enhances the Severity of Infection in Neutropenic Mice Orally Inoculated with Listeria monocytogenes EGD

C. J. Czuprynski1* and N. G. Faith1,2

Department of Pathobiological Sciences,1 School of Veterinary Medicine, and Food Research Institute, University of Wisconsin—Madison, Madison, Wisconsin2

Received 13 July 2001/ Accepted 1 November 2001

Epidemiological studies have suggested an association between antacid therapy and development of listeriosis in humans. In this study we used a neutropenic mouse model to demonstrate that oral administration of sodium bicarbonate shortly before intragastric (i.g.) inoculation with Listeria monocytogenes EGD (serotype 1/2a) significantly increased the severity of the resulting systemic infection. An explanation for this observation is provided by evidence that L. monocytogenes EGD is rapidly inactivated in synthetic gastric fluid at pH below 5. A second strain of L. monocytogenes (CM [serotype 1/2b]) exhibited little ability to cause systemic infection following i.g. inoculation and was not significantly enhanced by administration of sodium bicarbonate. Strain CM was readily inactivated in synthetic gastric fluid even at pH 7. These data suggest that gastric acidity and enzymes provide some innate defense against gastrointestinal listeriosis in neutropenic mice.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, 2015 Linden Dr. West, Madison, WI 53706. Phone and fax: (608) 262-8102. E-mail: czuprync{at}svm.vetmed.wisc.edu.

Clinical and Diagnostic Laboratory Immunology, March 2002, p. 477-481, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.477-481.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, H.-Y., Park, J.-H., Seok, S.-H., Baek, M.-W., Kim, D.-J., Lee, B.-H., Kang, P.-D., Kim, Y.-S., Park, J.-H. (2005). Potential antimicrobial effects of human lactoferrin against oral infection with Listeria monocytogenes in mice. J Med Microbiol 54: 1049-1054 [Abstract] [Full Text]  
  • Cotter, P. D., Hill, C. (2003). Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH. Microbiol. Mol. Biol. Rev. 67: 429-453 [Abstract] [Full Text]  
  • Czuprynski, C. J., Faith, N. G., Steinberg, H. (2002). Ability of the Listeria monocytogenes Strain Scott A To Cause Systemic Infection in Mice Infected by the Intragastric Route. Appl. Environ. Microbiol. 68: 2893-2900 [Abstract] [Full Text]  


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