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Clinical and Diagnostic Laboratory Immunology, March 2002, p. 499-502, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.499-502.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry

Lynn M. Herrmann,1* Timothy V. Baszler,2 Donald P. Knowles,1,2 and William P. Cheevers2

USDA Agricultural Research Service ADRU,1 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 991642

Received 3 October 2001/ Returned for modification 28 November 2001/ Accepted 18 December 2001

Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 x 106 DRLN cells, but were not detected in 3 x 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 x 106 PBLs.

* Corresponding author. Mailing address: USDA/ARS/ADRU, 3003 ADBF, Pullman, WA 99164. Phone: (509) 335-6068. Fax: (509) 335-8328. E-mail: lherrman{at}vetmed.wsu.edu.

Clinical and Diagnostic Laboratory Immunology, March 2002, p. 499-502, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.499-502.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Halliday, S., Houston, F., Hunter, N. (2005). Expression of PrPC on cellular components of sheep blood. J. Gen. Virol. 86: 1571-1579 [Abstract] [Full Text]  
  • Herrmann, L. M., Cheevers, W. P., Davis, W. C., Knowles, D. P., O'Rourke, K. I. (2003). CD21-Positive Follicular Dendritic Cells: A Possible Source of PrPSc in Lymph Node Macrophages of Scrapie-Infected Sheep. Am. J. Pathol. 162: 1075-1081 [Abstract] [Full Text]  
  • Hunter, N., Foster, J., Chong, A., McCutcheon, S., Parnham, D., Eaton, S., MacKenzie, C., Houston, F. (2002). Transmission of prion diseases by blood transfusion. J. Gen. Virol. 83: 2897-2905 [Abstract] [Full Text]  


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